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	Provedor de dados BABT (1) BJM (1) Autor Almeida,P.F. (1) Almeida,R.C.C. (1) Hofer,E. (1) Mall,Puspadhwaja (1) Mody,Rustom (1) Mohanty,Bijaya Kumar (1) Oliveira,I.C. (1) Patankar,Dhananjay Bhawanishankar (1) Tunga,Rashbehari (1) Mais... Palavra-chave Skimmed milk (2) Bacteriophage A511 (1) Detection (1) Lactococcus lactis (1) Laser light (1) Listeria (1) Nisin (1) Streptococcus agalactae (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment BJM Oliveira,I.C.; Almeida,R.C.C.; Hofer,E.; Almeida,P.F.. A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster... Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria; Bacteriophage A511; Laser light; Detection; Skimmed milk. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300040 Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440) BABT Mall,Puspadhwaja; Mohanty,Bijaya Kumar; Patankar,Dhananjay Bhawanishankar; Mody,Rustom; Tunga,Rashbehari. The influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440 was studied at shake flask level for 20 h. Media optimization (MRS broth) was studied to achieve enhanced growth of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v) with 0.15μg/ml of nisin supplemented with 0.5% (v/v) skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The production of nisin was affected by the physical parameters and maximum nisin production was at 30(0)C while... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nisin; Lactococcus lactis; Streptococcus agalactae; Skimmed milk. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100025 Registros recuperados: 2 Primeira ... 1 ... Última

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